Day 1

Morning Lecture:

Introduction PPT
Intro to Biotech PPT

Independent work:

Work on Personal page
Check Out:
Adventures in Synthetic Biology Comic book
Synthetic Biology explained (YouTube)
Decoding Synthetic Biology (YouTube)
The Promise and Perils of Synthetic Biology
Handmade Biology

Introduction to the lab

Lunch (11:00)

Lab (12:30):

Intro to micropipetting
DNA Necklaces
Procedures of Genetic Engineering
Gel animation

Homework:

Finish personal page

Day 2

Morning Lecture:

Intro to synthetic biology PPT
Engineering Design PPT
Playing God (history of genetic engineering--YouTube) We may do this later

Independent work:

Check out:
Adventures in Synthetic Biology Comic book
Synthetic Biology explained (YouTube)
Decoding Synthetic Biology (YouTube)
The Promise and Perils of Synthetic Biology
Handmade Biology
and other synbio resources on Resources page

Lunch

Lab (in classroom):

Design challenge PPT
BioBuilder Design Assignment

Homework:

Work on Ethics Response

Day 3

Morning Lecture:

Molecular Genetics Primer
Bozeman Biology: DNA and RNA - Part 1
Bozeman Biology: DNA and RNA - Part 2
McGraw-Hill Animation: DNA Replication
Bozeman Biology: DNA Replication
Bozeman Biology: Mutations

Protein Synthesis PPT_Autumn.ppt

Independent work:

Investigate Synbio ideas for research project. Use Resources and Activities wiki pages
Personal support time

Lunch

Lab:

PLasmid_extraction_manual.pdf

Homework:

Day 4

Morning Lecture:

Control and tuning of gene expression PPT
Epigenetics PPT
Gene regulation Robert Tjian

Independent work:

Investigation of epigenetics

Investigate Synbio ideas for research project. Use Resources and Activities wiki pages
Personal support time

Lunch

Lab:

Southern Blot
Southern Blot 2015

Homework:

Day 5

Morning Lecture:

Quorum sensing, two component system and bacterial photography PPT
Quorum Sensing Video
Bonnie Bassler--Tiny Conspiracies
Bonnie Bassler Ted Talk

Independent work:

Investigate Ethics and Safety links on SynBio, ethics and safety page.
Work on project

Lunch

Lab:

Finish southern Blot
Microarray simulation
Microarray on-line lab
Homework:

Day 6

Morning Lecture:

Chassis Design PowerPoint
Craig Venter synthetic life
George Church on Colbert

Independent work:

Work on project or ethics discussion

Lunch

Lab:

Fish protein electrophoresis
Seafood fraud
How seafood fraud works
If you eat fish...

Homework:

Day 7

Morning Lecture:

Genetic logic switches PPT

Independent work:

Work on project

Lunch

Lab:

Bacterial transformation
Kim's Bacterial transformation ppt
Bacterial Transformation lab Blue and Green

Homework:

Day 8

Morning Lecture:

Wired article Easy DNA editing will remake the world
What is CRSPR?
CRISPR Gene editing
America needs to figure out the ethics...
CRISPR could revolutionize ecosystem management
Ecological Implications of Synthetic Biology
Regulating Gene Drives
Gene Drives eLife article (click pdf icon on the upper right side of the page)
CRISPR Douda lecture
gene drives in mosquitos
CRISPR and human DNA
CRISPR Slides

Independent work:

Work on project

Lunch

Lab:

Finish Transformation
PCR Amplification Part 1
PCR PPT
PCR animation (YouTube)

Homework:

Day 9

Morning Lecture:

Independent work:

Work on project

Lunch

Lab:

PCR Amplification Part 2
ELISA
http://www.tccbiotech.org/files/documents/BioRad_ELISAmanual.pdf
https://www.youtube.com/watch?v=lNxZxJtvB94
ELISA Animation
ELISA Animation 2

Homework:

Day 10

Morning Lecture:

Field Trip Karp Lab
and MIT Museum

Return home late!!!!!

Independent work:

Lunch

Lab:

Homework:

Day 11

Morning Lecture:

Work on Project

Independent work:

Work on project

Lunch

Lab:

CRISPR Lab.pdf

CRISPR Lab.pdf


CRISPR lab link (easier than pdf)

CRISPR video from lab

Fish protein electrophoresis

Day 12

Morning Lecture:

Independent work:

Work on project

Lunch

Lab:

Finish CRISPR
Ion Exchange

Homework:

Day 13

Morning Lecture:

Project Presentations

Lunch

Lab:

Project Presentations

Day 14

Morning Lecture:

Project Presentation

Lunch

Lab:

Project Presentation

IGNORE BELOW:

Field Trip Broad Institute
Microarray

Synthetic Biology Regulation, Dr. Ken Oye MIT
Ecological Implications of Synthetic Biology
Regulating Gene Drives
Gene Drives eLife article (click pdf icon on the upper right side of the page)
Synbio Project.org
iGEM Safety article

Jay Keasling on Colbert
Jay Keasling lecture: Synthetic Biology Toward Biofuels
Jay Keasling on Artimisinin
Southern Blot
Southern Blot Animation
Creating Life, iGEM Documentary
Synthetic Biology and Metabolic Engineering Lecture


Bioinformatics Exercises 1-11
Codes for bioinformatics exercises 1, 4, and 5
NCBI
BLAST
GenBank
EMBOSS
Gene Cards
OMIM
ORF Finder
ReadSeq
BPROM
ClustalW

Scratch
TinkerCell download

DNA sequencing, bioinformatics and microarray lectures
Microarray animation
Third generation sequencing
Amanda's Bioinformatics presentation

Day 13

Morning Lecture:

Work on project

Independent work:

Work on project

Lunch

Lab:

Finish Southern Blot
Work on project

Homework:

PTCGenotyping.docx


PrimerDesignTutorial.docx


Bioinformatics Lesson 1.docx


bioinformatics codes-4.docx


Bioinformatics Exercise 2 and 3.docx


Bioinformatics exercise 4.docx


Bioinformatics Exercise 5.docx


Bioinformatics Exercise 6.docx


Bioinformatics Exercise 7.docx


Bioinformatics Exercise 8.docx


Bioinformatics Exercises 11and 12.docx